Genomic expression profiling of TNF- -treated BDC2.5 diabetogenic CD4+ T cells

Genomic expression profiling of TNF- -treated BDC2.5 diabetogenic CD4 T cells

TNFplays an important role in immune regulation, inflammation, and autoimmunity. Chronic TNF exposure has been shown to downmodulate T cell responses. In a mouse T cell hybridoma model, TNF attenuated T cell receptor (TCR) signaling. We have confirmed that chronic TNF and anti-TNF exposure suppressed and increased T cell responses, respectively. In adult TCR (BDC2.5) transgenic nonobese diabeti...

Cell Clones: A Central Role for TNF- Effector Function of Diabetogenic CD4 Th1 T

Effector function of diabetogenic CD4 Th1 T cell clones: a central role for TNF-alpha.

Effector function of T cells in autoimmune diabetes has been widely studied with mixed populations of lymphoid T cells stimulated ex vivo, but this approach does not permit evaluation of the contribution by a single T cell clone in the inflammatory site during pathogenesis. We have investigated cytokine production both in vitro and in vivo in a panel of diabetogenic CD4 Th1 T cell clones derive...

In vivo control of diabetogenic T-cells by regulatory CD4+CD25+ T-cells expressing Foxp3.

To understand the ability of regulatory T-cells to control diabetes development in clinically relevant situations, we established a new model of accelerated diabetes in young DP-BB rats by transferring purified T-cells from DR-BB rats made acutely diabetic. Transfer of 3, 5, 10, or 23 million pure in vitro-activated T-cells accelerated diabetes onset in >90% of the recipients, with the degree o...

AKT family and miRNAs expression in IL-2-induced CD4+T cells

Objective(s): Study of non-coding RNAs is considerable to elucidate principal biological questions or design new therapeutic strategies. miRNAs are a group of non-coding RNAs that their functions in PI3K/AKT signaling and apoptosis pathways after T cell activation is not entirely clear. Herein, miRNAs expression and their putative targets in the mentioned pathways were studied in the activated ...